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Lookup NU author(s): Emeritus Professor Peter Olive, Thanya Duangchinda, Elizabeth Ashforth, Emeritus Professor Alan Ward
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A net gain in long-chain polyunsaturated fatty acids (PUFA) occurs in Arenicola marina bioturbated mesocosms in the light and dark when brewery yeast is the only food. Fatty acid profiles were determined by GC-MS and quantified for system units. Bacterial fatty acid biosynthesis is indicated by accumulation of the bacterial fatty acids anteiso 14:0, iso 15:0, iso 16:0, anteiso 16:0, iso 17:0, C16:1n-7 and C18:1n-7 (cis-vaccenic acid). De novo production of longer chain PUFA within the worm tissues is implicated by a chain elongation converting linoleic acid (C18:2n-6) to C20:2n-6 and subsequent desaturation, resulting in a gain of arachidonic acid (C20:4n-6) and eicosapentaenoic acid (C20:5n-3) via the intermediary C20:3n-6. To test for the presence of PUFA-synthesising bacteria in system units, 495 cloned 16S rDNA fragments were compared with the databases and degenerate PCR primers were designed for the ketoacyl acyl carrier protein synthase gene highly conserved in the polyketide synthase region of known PUFA-synthesising Gammaproteobacteria. Gammaproteobacteria related to known PUFA producers were not abundant (
Author(s): Olive PJW, Duangchinda T, Ashforth E, Craig S, Ward AC, Davies SJ
Publication type: Article
Publication status: Published
Journal: Marine Ecology - Progress Series
Year: 2009
Volume: 387
Pages: 223-239
Print publication date: 01/01/2009
ISSN (print): 0171-8630
ISSN (electronic): 1616-1599
Publisher: Inter-Research
URL: http://dx.doi.org/10.3354/meps08088
DOI: 10.3354/meps08088
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