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Expression and modulation of TUB by insulin and thyroid hormone in primary rat and murine 3T3-L1 adipocytes

Lookup NU author(s): Dr Gary Litherland

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Abstract

tub encodes a protein of poorly understood function, but one implicated strongly in the control of energy balance and insulin sensitivity. Whilst tub expression is particularly prominent in neurones it is also detectable in extraneuronal tissues. We show here, for the first time, expression of TUB protein in rat adipocytes and the murine adipocyte model 3T3-L1 and demonstrate that insulin induces its tyrosine phosphorylation and association with the insulin receptor. TUB expression is regulated developmentally during adipogenic differentiation of 3T3-L1 cells and in response to cell treatment with thyroid hormone or induction of insulin resistance. TUB was upregulated 5- to 10-fold in adipocytes from obese Zucker rats and 3T3-L1 adipocytes that had been rendered insulin resistant, a response that could be antagonised by rosiglitasone, an insulin-sensitising drug. Our data are consistent with a previously unforeseen role for TUB in insulin signalling and fuel homeostasis in adipocytes. © 2009 Elsevier Inc. All rights reserved.

Publication metadata

Author(s): Stretton C, Litherland G, Moynihan A, Hajduch E, Hundal H

Publication type: Article

Publication status: Published

Journal: Biochemical and Biophysical Research Communications

Year: 2009

Volume: 390

Issue: 4

Pages: 1328-1333

ISSN (print): 0006-291X

ISSN (electronic): 1090-2104

Publisher: Academic Press

URL: http://dx.doi.org/10.1016/j.bbrc.2009.10.147

DOI: 10.1016/j.bbrc.2009.10.147

PubMed id: 19887065

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