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Development of screening assays and discovery of initial inhibitors of pneumococcal peptidoglycan deacetylase PgdA

Lookup NU author(s): Dr Nhat Khai Bui, Flint Stevenson-Jones, Benjamin Zeuch, Professor Waldemar Vollmer

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Abstract

The essential cell wall peptidoglycan is the target of several components of the innate immune system and its disruption results in lysis of invading bacteria. The pathogen Streptococcus pneumoniae produces a peptidoglycan N-acetylglucosamine deacetylase, PgdA, to modify the peptidoglycan structure. The activity of PgdA contributes to the bacteria's resistance to lysozyme, which is an important antimicrobial factor of the human innate immune system. In this study we report on the activity of PgdA against natural and artificial substrates. We have also established a virtual high-throughput screening and a new enzyme assay to search for compounds inhibiting PgdA. Two compounds with IC50 values in the micromolar range have been identified and they could serve as leads for the search of inhibitors of PgdA, an important pneumococcal virulence factor. (C) 2011 Elsevier Inc. All rights reserved.

Publication metadata

Author(s): Bui NK, Turk S, Buckenmaier S, Stevenson-Jones F, Zeuch B, Gobec S, Vollmer W

Publication type: Article

Publication status: Published

Journal: Biochemical Pharmacology

Year: 2011

Volume: 82

Issue: 1

Pages: 43-52

Print publication date: 08/04/2011

ISSN (print): 0006-2952

ISSN (electronic): 1873-2968

Publisher: Elsevier Inc.

URL: http://dx.doi.org/10.1016/j.bcp.2011.03.028

DOI: 10.1016/j.bcp.2011.03.028

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