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Lookup NU author(s): Dr David Cox, Dr Claudia RaccaORCiD
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In hippocampal neurons, AMPA receptors (AMPARs) mediate fast excitatory postsynaptic responses at glutamatergic synapses, and are involved in various forms of synaptic plasticity. Dendritic local protein synthesis of selected AMPAR subunit mRNAs is considered an additional mechanism to independently and rapidly control the strength of individual synapses. We have used fluorescent in situ hybridisation and immunocytochemistry to analyse the localisation of AMPAR subunit (GluA1-4) mRNAs and their relationship with the translation machinery in principal cells and interneurons of the adult rat hippocampus. The mRNAs encoding all four AMPAR subunits were detected in the somata and dendrites of CA3 and CA1 pyramidal cells and those of six classes of CA1 GABAergic interneurons. GluA1-4 subunit mRNAs were highly localised to the apical dendrites of pyramidal cells, while in interneurons they were present in multiple dendrites. In contrast, in dentate gyrus, GluA1-4 subunit mRNAs were virtually restricted to the somata and absent from the dendrites of granule cells. These different regional and cell type-specific labelling patterns also correlated with the localisation of markers for components of the protein synthesis machinery. Our results support the local translation of GluA1-4 mRNAs in dendrites of hippocampal pyramidal cells and CA1 interneurons but not in granule cells of the dendate gyrus. Furthermore, the regional and cell type-specific differences we observed suggest that each cell type uses distinct ways of regulating the local translation of AMPA receptor subunits
Author(s): Cox DJ, Racca C
Publication type: Article
Publication status: Published
Journal: Journal of Comparative Neurology
Year: 2013
Volume: 521
Issue: 9
Pages: 1954-2007
Print publication date: 18/04/2013
ISSN (print): 0021-9967
ISSN (electronic): 1096-9861
Publisher: John Wiley & Sons, Inc.
URL: http://dx.doi.org/10.1002/cne.23292
DOI: 10.1002/cne.23292
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