ePrints

Profiling shifts in protein complement in tomato fruit induced by atmospheric ozone-enrichment and/or wound-inoculation with Botrytis cinerea

Lookup NU author(s): Nicolaos Tzortzakis, Dr Tahar Taybi, Edna Antony, Dr Ian Singleton, Emerita Professor Anne Borland, Emeritus Professor Jerry Barnes

Downloads

Full text for this publication is not currently held within this repository. Alternative links are provided below where available.

Abstract

To unravel the mechanism by which low level atmospheric ozone-enrichment (0.05 μmol mol−1) increases the shelf-life of tomato fruit (Lycopersicon esculentum Mill.) by suppressing the growth of pathogens (Botrytis cinerea), protein yield and composition were examined during and following exposure to the gas at 13 °C/95% RH. Ozone-enrichment caused marked changes in protein yield and composition in control tomato fruit and suppressed shifts in the proteome induced by wounding/fungal attack. Wound/fungal-inoculation with B. cinerea resulted in a 7% increase in protein yield, and the down-regulation of at least 32 proteins. A number of proteins affected under ozone and wound/fungal-inoculation treatments are involved in the control of cellular oxidative status. Proteins that may be enhanced under oxidative stress were induced during ozone exposure (e.g. thioredoxin peroxidase-TPX), but suppressed following transfer to ‘clean air’ (e.g. ascorbate peroxidase-APX1). Constitutively-expressed proteins tended to increase reversibly under ozone-treatment, however proteins involved in ripening such as an enzyme related to ethylene biosynthesis (1-aminocyclopropane-1-carboxylate oxidase-ACO) were markedly reduced in ozone-treated tomato fruit but increased in wound-inoculated fruit. Levels of proteins involved in carbohydrate metabolism, pentose phosphate pathway, terpenoid and flavonoid biosynthesis differentiated among the treatments. The presented dataset makes a central contribution to a comprehensive analysis of the manner in which tomato fruit react to ozone-enrichment and/or pathogen infection during storage/transit.

Publication metadata

Author(s): Tzortzakis N, Taybi T, Antony E, Singleton I, Borland A, Barnes J

Publication type: Article

Publication status: Published

Journal: Postharvest Biology and Technology

Year: 2013

Volume: 78

Pages: 67-75

Print publication date: 01/04/2013

ISSN (print): 0925-5214

ISSN (electronic): 1873-2356

Publisher: Elsevier

URL: http://dx.doi.org/10.1016/j.postharvbio.2012.12.005

DOI: 10.1016/j.postharvbio.2012.12.005

Altmetrics

Share