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Lookup NU author(s): Sridhar Govindarajan, Emeritus Professor Calum McNeilORCiD
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Glutamate mediates most of the excitatory synaptic transmission in the brain, and its abnormal regulation is considered a key factor underlying the appearance and progression of many neurodegenerative and psychiatric diseases. In this work, a microdisc-based amperometric biosensor for glutamate detection with highly enhanced selectivity and good stability is proposed. The biosensor utilizes the enzyme glutamate oxidase which was dip-coated onto 125 μm diameter platinum discs. To improve selectivity, phosphatidylethanolamine was pre-coated prior to enzyme deposition, and electropolymerization of o-phenylenediamine was performed to entrap the enzyme within a polymer matrix. A variety of coating configurations were tested in order to optimize biosensor performance. For stability measurements, biosensors were biased continuously and calibration curves calculated each day for a period of 5–6 days. The optimized biosensors exhibited very high sensitivity (71 ± 1 mA M−1 cm−2), low detection limit of ∼2.5 μM glutamate, selectivity (over 87% against ascorbic acid), very good temporal stability during continuous use, and a response time of <5 s. These biosensors are therefore good candidates for further development as devices for continuous monitoring during traumatic brain injury or neurosurgery.
Author(s): Govindarajan S, McNeil CJ, Lowry JP, McMahon CP, O'Neill RD
Publication type: Article
Publication status: Published
Journal: Sensors and Actuators B: Chemical
Year: 2013
Volume: 178
Pages: 606–614
Print publication date: 16/01/2013
ISSN (print): 0925-4005
ISSN (electronic): 1873-3077
Publisher: Elsevier
URL: http://dx.doi.org/10.1016/j.snb.2012.12.077
DOI: 10.1016/j.snb.2012.12.077
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