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Biocompatibility and enhanced osteogenic differentiation of human mesenchymal stem cells in response to surface engineered poly(D,L-lactic-co-glycolic acid) microparticles

Lookup NU author(s): Professor David Deehan, Callie Knuth, Dr Rachel Oldershaw

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Abstract

Tissue engineering strategies can be applied to enhancing osseous integration of soft tissue grafts during ligament reconstruction. Ligament rupture results in a hemarthrosis, an acute intra-articular bleed rich in osteogenic human mesenchymal stem cells (hMSCs). With the aim of identifying an appropriate biomaterial with which to combine hemarthrosis fluid-derived hMSCs (HF-hMSCs) for therapeutic application, this work has investigated the biocompatibility of microparticles manufactured from two forms of poly(D, L-lactic-co-glycolic acid) (PLGA), one synthesized with equal monomeric ratios of lactic acid to glycolic acid (PLGA 50: 50) and the other with a higher proportion of lactic acid (PLGA 85: 15) which confers a longer biodegradation time. The surfaces of both types of microparticles were functionalized by plasma polymerization with allylamine to increase hydrophilicity and promote cell attachment. HF-hMSCs attached to and spread along the surface of both forms of PLGA microparticle. The osteogenic response of HF-hMSCs was enhanced when cultured with PLGA compared with control cultures differentiated on tissue culture plastic and this was independent of the type of polymer used. We have demonstrated that surface engineered PLGA microparticles are an appropriate biomaterial for combining with HF-hMSCs and the selection of PLGA is relevant only when considering the biodegradation time for each biomedical application. (C) 2013 Wiley Periodicals, Inc.

Publication metadata

Author(s): Rogers CM, Deehan DJ, Knuth CA, Rose FRAJ, Shakesheff KM, Oldershaw RA

Publication type: Article

Publication status: Published

Journal: Journal of Biomedical Materials Research Part A

Year: 2014

Volume: 102

Issue: 11

Pages: 3872-3882

Print publication date: 01/11/2014

Online publication date: 20/12/2013

Acceptance date: 09/12/2013

ISSN (print): 1549-3296

ISSN (electronic): 1552-4965

Publisher: Wiley-Blackwell

URL: http://dx.doi.org/10.1002/jbm.a.35063

DOI: 10.1002/jbm.a.35063

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