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Characterization of the LM5 pectic galactan epitope with synthetic analogues of β-1,4-d-galactotetraose

Lookup NU author(s): Professor William WillatsORCiD

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Abstract

Plant cell wall glycans are important polymers that are crucial to plant development and serve as an important source of sustainable biomass. The study of polysaccharides in the plant cell wall relies heavily on monoclonal antibodies (mAbs) for localization and visualization of glycans, using e.g. immunofluorescent microscopy. Here, we describe the detailed epitope mapping of the mAb LM5 that is shown to bind to a minimum of three sugar residues at the non-reducing end of linear beta-1,4-linked galactan. The study uses de novo synthetic analogues of galactans combined with carbohydrate microarray and competitive inhibition ELISA for analysis of antibody-carbohydrate interactions.

Publication metadata

Author(s): Andersen MCF, Boos I, Marcus SE, Kračun SK, Rydahl MG, Willats WGT, Knox JP, Clausen MH

Publication type: Article

Publication status: Published

Journal: Carbohydrate Research

Year: 2016

Volume: 436

Pages: 36-40

Print publication date: 21/12/2016

Online publication date: 30/10/2016

Acceptance date: 27/10/2016

ISSN (print): 0008-6215

ISSN (electronic): 1873-426X

Publisher: Pergamon Press

URL: http://dx.doi.org/10.1016/j.carres.2016.10.012

DOI: 10.1016/j.carres.2016.10.012

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