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Extraordinary long-stem confers resistance of intrinsic terminators to processive antitermination

Lookup NU author(s): Dr Ling Juan Wu

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Abstract

© The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research. Many prokaryotic operons encode a processive antitermination (P-AT) system. Transcription complexes associated with an antitermination factor can bypass multiple transcription termination signals regardless of their sequences. However, to avoid compromising transcriptional regulation of downstream regions, the terminator at the end of the operon needs to be resistant to antitermination. So far, no studies on the mechanism of resistance to antitermination have been reported. The recently discovered conAn P-AT system is composed of two components that are encoded at the start of many conjugation operons on plasmids of Gram-positive bacteria. Here we report the identification of a conAn-resistant terminator, named TerR, in the conjugation operon of the Bacillus subtilis plasmid pLS20, re-defining the end of the conjugation operon. We investigated the various characteristics of TerR and show that its extraordinary long stem is the determining feature for resistance to antitermination. This is the first P-AT resistance mechanism to be reported.

Publication metadata

Author(s): Miguel-Arribas A, Martin-Maria A, Alaerds ECW, Val-Calvo J, Yuste L, Rojo F, Abia D, Wu LJ, Meijer WJJ

Publication type: Article

Publication status: Published

Journal: Nucleic Acids Research

Year: 2023

Volume: 51

Issue: 12

Pages: 6073-6086

Print publication date: 07/07/2023

Online publication date: 01/05/2023

Acceptance date: 20/04/2023

ISSN (print): 0305-1048

ISSN (electronic): 1362-4962

Publisher: Oxford University Press

URL: https://doi.org/10.1093/nar/gkad333

DOI: 10.1093/nar/gkad333

PubMed id: 37125647

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