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Lookup NU author(s): Tom PitherORCiD, Dr Lu Wang, Lucy BatesORCiD, Dr Morvern Morrison, Catriona Charlton, Chelsea Griffiths, Jamie Macdonald, Dr Venetia BigleyORCiD, Maria Mavridou, Joseph Barsby, Dr Lee Borthwick, Professor John Dark, Dr Bill ScottORCiD, Professor Simi Ali, Professor Andrew FisherORCiD
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
Copyright © 2023 The Author(s). Published by Wolters Kluwer Health, Inc. BACKGROUND: The association between interleukin-1β (IL-1β) concentrations during ex vivo lung perfusion (EVLP) with donor organ quality and post-lung transplant outcome has been demonstrated in several studies. The mechanism underlying IL-1β-mediated donor lung injury was investigated using a paired single-lung EVLP model. METHODS: Human lung pairs were dissected into individual lungs and perfused on identical separate EVLP circuits, with one lung from each pair receiving a bolus of IL-1β. Fluorescently labeled human neutrophils isolated from a healthy volunteer were infused into both circuits and quantified in perfusate at regular timepoints. Perfusates and tissues were subsequently analyzed, with perfusates also used in functional assays. RESULTS: Neutrophil numbers were significantly lower in perfusate samples collected from the IL-1β-stimulated lungs consistent with increased neutrophil adhesion ( P = 0.042). Stimulated lungs gained significantly more weight than controls ( P = 0.046), which correlated with soluble intercellular adhesion molecule-1 (R 2 = 0.71, P = 0.0043) and von-Willebrand factor (R 2 = 0.39, P = 0.040) in perfusate. RNA expression patterns for inflammatory genes were differentially regulated via IL-1β. Blockade of IL-1β significantly reduced neutrophil adhesion in vitro ( P = 0.025). CONCLUSION: These data illustrate the proinflammatory functions of IL-1β in the context of EVLP, suggesting this pathway may be susceptible to therapeutic modulation before transplantation.
Author(s): Pither T, Wang L, Bates L, Morrison M, Charlton C, Griffiths C, Macdonald J, Bigley V, Mavridou M, Barsby J, Borthwick L, Dark J, Scott W, Ali S, Fisher AJ
Publication type: Article
Publication status: Published
Journal: Transplantation
Year: 2023
Volume: 107
Issue: 10
Pages: 2179-2189
Print publication date: 01/10/2023
Acceptance date: 23/02/2023
Date deposited: 11/10/2023
ISSN (print): 0041-1337
ISSN (electronic): 1534-6080
Publisher: Wolters Kluwer Health, Inc.
URL: https://doi.org/10.1097/TP.0000000000004613
DOI: 10.1097/TP.0000000000004613
PubMed id: 37143202
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