Monitoring the Interaction of the Peptidoglycan with the Bacterial β-Barrel Assembly Machinery

Corona, F; Vollmer, W

doi:10.1007/978-1-0716-3734-0_11

Monitoring the Interaction of the Peptidoglycan with the Bacterial β-Barrel Assembly Machinery

Lookup NU author(s): Federico Corona, Professor Waldemar Vollmer

Downloads

Full text for this publication is not currently held within this repository. Alternative links are provided below where available.

Abstract

© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. Gram-negative bacteria coordinate the biosynthesis of their different cell envelope components. Growth of the outer membrane (OM) requires the essential β-barrel assembly machine (BAM), which inserts OM proteins (OMPs) into the OM. The underlying peptidoglycan (PG) sacculus grows by the insertion of nascent glycan chains. We have previously identified interactions between BAM and PG in E. coli and showed that these interactions coordinate OM biogenesis with PG growth. BAM responds to the maturation state of the PG, and this mechanism activates preferentially BAM complexes at sites of active PG synthesis. Here we present protocols to purify soluble Bam proteins and full-length BamABCDE, isolate PG and soluble PG fragments, and study BAM-PG interactions with the isolated components. We also describe the protocol to detect interactions between Bam proteins and PG in cells.