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Analysis of pigeon intestinal mucin allergens using a novel dot blot assay

Lookup NU author(s): Professor Christopher Baldwin, Emerita Professor Jane Calvert

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Abstract

Many glycoproteins contain multiple glycosylation sites that can present multi-valent epitopes for antigenic recognition. Release of the oligosaccharides results in loss of avidity of antibody binding, which has been overcome by reforming clustered ligands, usually by reductive amination of free reducing oligosaccharides to poly-amine groups. We have adapted this approach to hydrazinolytic release of O-linked chains of mucin glycoproteins and 'one-pot' microscale coupling to poly-L-lysine (PLL). The conjugated PLL adheres to nitrocellulose membranes through washing procedures required for antibody or lectin overlay and detection. We show evidence for the applicability of this technique using lectin and antibody reactivity to the oligosaccharides of pigeon intestinal mucins, which have been implicated in the allergic disease pigeon fanciers' lung. (C) 2000 Elsevier Science Ltd.


Publication metadata

Author(s): Baldwin CI, Calvert JE, Renouf DV, Kwok C, Hounsell EF

Publication type: Article

Publication status: Published

Journal: Carbohydrate Research

Year: 2000

Volume: 326

Issue: 1

Pages: 43-49

ISSN (print): 0008-6215

ISSN (electronic): 1873-426X

Publisher: Pergamon

URL: http://dx.doi.org/10.1016/S0008-6215(00)00023-9

DOI: 10.1016/S0008-6215(00)00023-9

PubMed id: 16001505


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