Platelet Function Following Acute Cerebral Ischemia

  1. Lookup NU author(s)
  2. Dr Jonathan Smout
  3. Dr Alexander Dyker
  4. Professor Gary Ford
  5. Dr Patrick Kesteven
  6. Professor Gerard Stansby
Author(s)Smout J, Dyker A, Cleanthis M, Ford G, Kesteven P, Stansby G
Publication type Article
JournalAngiology
Year2009
Volume60
Issue3
Pages362-369
ISSN (print)0003-3197
ISSN (electronic)1940-1574
Full text for this publication is not currently held within this repository. Alternative links are provided below where available.
Background: Studies have previously identified increased levels of platelet activation following acute ischemic stroke. In order to evaluate new antiplatelet agents and their combinations, there is a need for accurate measures of platelet activation. Methods: Blood was taken from 17 patients within 24 hours of an acute ischemic stroke, and then at 3, 7, 14 and 42 days. For comparison, a group of 18 stable arteriopaths had identical tests performed. Platelet aggregation was measured using a free platelet counting technique, and platelet surface P-selectin and monocyte platelet aggregates (MPAs) were measured using flow cytometry. Soluble P-selectin and D-dimers were measured by an enzyme linked immune assay. Results: The initial level of MPAs was significantly raised in the stroke patients compared with the stable patients (p = 0.04, 14.2% vs. 9.3%); however, this difference was not significantly higher than later study points (14.2%, 10.1%, 9.3%, 11.9%, 11.3%; days 1, 3, 7, 14 and 42 respectively. Day 1 vs. day 7 p = 0.07 ANOVA). No changes in P-selectin or platelet aggregation were identified. D-dimer levels were significantly higher on day 7 than day 42 (p < 0.01), and fibrinogen levels were elevated on both days 3 and 14 compared with day 42. Fibrinogen levels were not elevated compared with stable patients. Conclusions: MPA levels are elevated following an acute ischemic stroke compared to stable patients, but no significant change was seen with other platelet markers. This study suggests MPAs are a more sensitive marker of platelet activation than either P-selectin or aggregation.
PublisherSage Publications, Inc.
URLhttp://dx.doi.org/10.1177/0003319709332959
DOI10.1177/0003319709332959
Actions    Link to this publication