The Expression and Function of the NKRP1 Receptor Family in C57BL/6 Mice

  1. Lookup NU author(s)
  2. Dr Jonathan Aust
  3. Frances Davison
  4. Dr Katarzyna Mickiewicz
  5. Professor Colin Brooks
Author(s)Aust JG, Gays F, Mickiewicz KM, Buchanan E, Brooks CG
Publication type Article
JournalJournal of Immunology
Year2009
Volume183
Issue1
Pages106-116
ISSN (print)0022-1767
ISSN (electronic)1550-6606
Full text for this publication is not currently held within this repository. Alternative links are provided below where available.
NKRP1 receptors were discovered more than 20 years ago, but due to a lack of appropriate reagents, our understanding of them has remained limited. Using a novel panel of mAbs that specifically recognize mouse NKRP1A, D, and F molecules, we report here that NKRP1D expression is limited to a subpopulation of NK cells, but in contrast to Ly49 receptors appears to be expressed in a normal codominant manner. NKRP1D(-) and NKRP1D(+) NK cells are functionally distinct, NKRP1D(+) cells showing reduced expression of various Ly49 receptors, elevated expression of CD94/NKG2 receptors, and higher IFN-gamma secretion and cytotoxicity than NKRP1D(-) cells. Furthermore, NKRP1D(+) NK cells were unable to kill transfected cells expressing high levels of Clr-b molecules, but readily killed MHC class-I-deficient blast cells that express only low levels of Clr-b. NKRP1A and NKRP1F were expressed at low levels on all splenic and bone marrow NK cells, but mAb-induced cross-linking of NKRP1A and NKRP1F caused no significant enhancement or inhibition of NK cell cytotoxicity and no detectable production of IFN-gamma. NKRP1A, D, and F expression could not be detected on NKT cells, all of which express NKRP1C, and although some activated T cells expressed NKRP1C and perhaps low levels of NKRP1A, no significant expression of NKRP1D or F could be detected. NKRP1 molecules expressed on NK cells or transfectants were down-regulated by cross-linking with mAbs or cell surface ligands, and using this phenomenon as a functional assay for NKRP1-ligand interaction revealed that NKRP1F can recognize CLR-x.
PublisherAmerican Association of Immunologists
URLhttp://dx.doi.org/10.4049/jimmunol.0804281
DOI10.4049/jimmunol.0804281
NotesJournal of immunology (Baltimore, Md. : 1950)
Actions    Link to this publication
Share