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Differential fate of erythromycin and beta-lactam resistance genes from swine lagoon waste under different aquatic conditions
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Dr Charles Knapp
Professor David Graham
Knapp CW, Zhang W, Sturm BSM, Graham DW
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The attenuation and fate of erythromycin-resistance-methylase (
) and extended-spectrum beta-lactamse (
) genes were quantified over time in aquatic systems by adding 20-L swine waste to 11,300-L outdoor mesocosms that simulated receiving water conditions below intensive agricultural operations. The units were prepared with two different light-exposure scenarios and included artificial substrates to assess gene movement into biofilms. Of eleven genes tested, only
were found in sufficient quantity for monitoring. The genes disappeared rapidly from the water column and first-order water-column disappearance coefficients were calculated. However, detected gene levels became elevated in the biofilms within 2 days, but then disappeared overtime. Differences were observed between sunlight and dark treatments and among individual genes, suggesting that ecological and gene-specific factors play roles in the fate of these genes after release into the environment. Ultimately, this information will aid in generating better predictive models for gene fate.
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