A cost-effective, simple and high-throughput method for DNA extraction from insects
- Lookup NU author(s)
- Dr Malgorzata Lagisz
- Dr Gordon Port
- Dr Kirsten Wolff
|
|
|
|
| Author(s) | | Lagisz M, Port G, Wolff K |
| Publication type | | Article |
| Journal | | Insect Science |
| Year | | 2010 |
| Volume | | 17 |
| Issue | | 5 |
| Pages | | 465-470 |
| ISSN (print) | | 1672-9609 |
| ISSN (electronic) | | 1744-7917 |
|
|
|
| Full text for this publication is not currently held within this repository. Alternative links are provided below where available. |
|
|
|
|
| We present a high-throughput cost-effective method to extract DNA suitable for polymerase chain reaction (PCR) from insect tissue. The method uses standard 200 μL-deep 96-well plates in which samples are ground, digested and subsequently purified. The test extraction using four different insect species and controlling for potential contamination showed that the method yields good-quantity and quality DNA. PCR with mitochondrial and nuclear primers was reliable. The proposed extraction protocol combines the speed of commercial 96-well plate methods with the economies associated with readily available and cheap laboratory chemicals, consumables and equipment. Therefore, this method is particularly suitable for low-budget research projects and for laboratories with only basic equipment present. |
|
|
|
| Publisher | | Wiley-Blackwell |
| URL | | http://dx.doi.org/10.1111/j.1744-7917.2010.01318.x |
| DOI | | 10.1111/j.1744-7917.2010.01318.x |
|
|
Actions | |   |
