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The structure of Yersinia pestis Caf1 polymer in free and adjuvant bound states

Lookup NU author(s): Dr Andrei Soliakov, Professor Robin Harris, Professor Jeremy LakeyORCiD

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Abstract

Caf1 of the plague bacterium, Yersinia pestis is a polymeric virulence factor and vaccine component. formed from monomers by a donor strand exchange (DSE) mechanism. Here. EM images of Caf1 reveal flexible polymers up to 1 5 mu m long (4 MDa). The bead-like structures along the polymer are 58 +/- 1 nm long and correspond to single Caf1 proteins. Short polymers often form circles, presumably by DSE. We also provide the first images of proteins bound to alhydrogel adjuvant. Call, hemocyanin and anthrax PA are all resolved clearly and Caf1 exhibits adjuvant bound stretches with long intervening loops draped from the edges. (C) 2010 Elsevier Ltd. All rights reserved.

Publication metadata

Author(s): Soliakov A, Harris JR, Watkinson A, Lakey JH

Publication type: Article

Publication status: Published

Journal: Vaccine

Year: 2010

Volume: 28

Issue: 35

Pages: 5746-5754

Print publication date: 01/08/2010

ISSN (print): 0264-410X

ISSN (electronic): 1873-2518

Publisher: Elsevier

URL: http://dx.doi.org/10.1016/j.vaccine.2010.05.074

DOI: 10.1016/j.vaccine.2010.05.074

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