The p.M292T NDUFS2 mutation causes complex I-deficient Leigh syndrome in multiple families

  1. Lookup NU author(s)
  2. Dr Helen Tuppen
  3. Dr Vanessa Hogan
  4. Dr Langping He
  5. Dr Mazhor Aldosary
  6. Dr Gabriele Saretzki
  7. Charlotte Alston
  8. Dr Andrew Morris
  9. Professor Zofia Chrzanowska-Lightowlers
  10. Dr Bobby McFarland
  11. Professor Robert Taylor
Author(s)Tuppen HA, Hogan VE, He L, Blakely EL, Worgan L, Al-Dosary M, Saretzki G, Alston CL, Morris AA, Clarke M, Jones S, Devlin AM, Mansour S, Chrzanowska-Lightowlers ZMA, Thorburn DR, McFarland R, Taylor RW
Publication type Article
JournalBrain
Year2010
Volume133
Issue10
Pages2952-2963
ISSN (print)0006-8950
ISSN (electronic)1460-2156
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Isolated complex I deficiency is the most frequently observed oxidative phosphorylation defect in children with mitochondrial disease, leading to a diverse range of clinical presentations, including Leigh syndrome. For most patients the genetic cause of the biochemical defect remains unknown due to incomplete understanding of the complex I assembly process. Nonetheless, a plethora of pathogenic mutations have been described to date in the seven mitochondrial-encoded subunits of complex I as well as in 12 of the nuclear-encoded subunits and in six assembly factors. Whilst several mitochondrial DNA mutations are recurrent, the majority of these mutations are reported in single families. We have sequenced core structural and functional nuclear-encoded subunits of complex I in a cohort of 34 paediatric patients with isolated complex I deficiency, identifying pathogenic mutations in 6 patients. These included a novel homozygous NDUFS1 mutation in an Asian child with Leigh syndrome, a previously identified NDUFS8 mutation (c.236C>T, p.P79L) in a second Asian child with Leigh-like syndrome and six novel, compound heterozygous NDUFS2 mutations in four white Caucasian patients with Leigh or Leigh-like syndrome. Three of these children harboured an identical NDUFS2 mutation (c.875T>C, p.M292T), which was also identified in conjunction with a novel NDUFS2 splice site mutation (c.866+4A>G) in a fourth Caucasian child who presented to a different diagnostic centre, with microsatellite and single nucleotide polymorphism analyses indicating that this was due to an ancient common founder event. Our results confirm that NDUFS2 is a mutational hotspot in Caucasian children with isolated complex I deficiency and recommend the routine diagnostic investigation of this gene in patients with Leigh or Leigh-like phenotypes.
PublisherOxford University Press
URLhttp://dx.doi.org/10.1093/brain/awq232
DOI10.1093/brain/awq232
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