Dispersal of Biofilms by Secreted, Matrix Degrading, Bacterial DNase

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  2. Dr Michael Hall
  3. Professor Grant Burgess
Author(s)Nijland N, Hall MJ, Burgess JG
Publication type Article
JournalPLoS One
Year2010
Volume5
Issue12
Pages
ISSN (electronic)1932-6203
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Microbial biofilms are composed of a hydrated matrix of biopolymers including polypeptides, polysaccharides and nucleic acids and act as a protective barrier and microenvironment for the inhabiting microbes. While studying marine biofilms, we observed that supernatant produced by a marine isolate of Bacillus licheniformis was capable of dispersing bacterial biofilms. We investigated the source of this activity and identified the active compound as an extracellular DNase (NucB). We have shown that this enzyme rapidly breaks up the biofilms of both Gram-positive and Gram-negative bacteria. We demonstrate that bacteria can use secreted nucleases as an elegant strategy to disperse established biofilms and to prevent de novo formation of biofilms of competitors. DNA therefore plays an important dynamic role as a reversible structural adhesin within the biofilm.
PublisherPublic Library of Science
URLhttp://dx.doi.org/10.1371/journal.pone.0015668
DOI10.1371/journal.pone.0015668
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