Single-sweep voltage sensitive dye imaging of interacting identified neurons

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  2. Dr Wolfgang Stein
  3. Dr Peter Andras
Author(s)Stein W, St├Ądele C, Andras P
Publication type Article
JournalJournal of Neuroscience Methods
Year2011
Volume194
Issue2
Pages224-234
ISSN (print)0165-0270
ISSN (electronic)1872-678X
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The simultaneous recording of many individual neurons is fundamental to understanding the integral functionality of neural systems. Imaging with voltage-sensitive dyes (VSDs) is a key approach to achieve this goal and a promising technique to supplement electrophysiological recordings. However, the lack of connectivity maps between imaged neurons and the requirement of averaging over repeated trials impede functional interpretations. Here we demonstrate fast, high resolution and single-sweep VSD imaging of identified and synaptically interacting neurons. We show for the first time the optical recording of individual action potentials and mutual inhibitory synaptic input of two key players in the well-characterized pyloric central pattern generator in the crab stomatogastric ganglion (STG). We also demonstrate the presence of individual synaptic potentials from other identified circuit neurons. We argue that imaging of neural networks with identifiable neurons with well-known connectivity, like in the STG, is crucial for the understanding of emergence of network functionality.
PublisherElsevier BV
URLhttp://dx.doi.org/10.1016/j.jneumeth.2010.10.007
DOI10.1016/j.jneumeth.2010.10.007
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