Raised interleukin-17 is immuno-localised to neutrophils in cystic fibrosis lung disease
- Lookup NU author(s)
- Dr Malcolm Brodlie
- Dr Michael McKean
- Dr Amy Anderson
- Dr Catharien Hilkens
- Professor Andrew Fisher
- Professor Paul Corris
- Dr James Lordan
- Dr Christopher Ward
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| Author(s) | | Brodlie M, McKean MC, Johnson GE, Anderson AE, Hilkens CM, Fisher AJ, Corris PA, Lordan JL, Ward C |
| Publication type | | Article |
| Journal | | European Respiratory Journal |
| Year | | 2010 |
| Volume | | 37 |
| Issue | | 6 |
| Pages | | 1378-1385 |
| ISSN (print) | | 0903-1936 |
| ISSN (electronic) | | 1399-3003 |
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| Full text for this publication is not currently held within this repository. Alternative links are provided below where available. |
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| Abstract Interleukin-17 is pivotal in orchestrating the activity of neutrophils. Neutrophilic inflammation is the dominant pathology in cystic fibrosis lung disease. We investigated interleukin-17 protein expression in the lower airway in cystic fibrosis, its cellular immuno-localisation and the effects of interleukin-17 on cystic fibrosis primary bronchial epithelial cells. Immunohistochemistry was performed on explanted cystic fibrosis lungs and compared to the non-suppurative condition pulmonary hypertension. Airway lavages and epithelial cultures were generated from explanted cystic fibrosis lungs. Immunoreactivity for interleukin-17 was significantly increased in the lower airway epithelium in cystic fibrosis (median 14.1%) compared to pulmonary hypertension (2.95%, P=0.0001). The number of cells staining positive for interleukin-17 in the lower airway mucosa was also increased (64 compared to 9/mm basement membrane, P=0.0005) and included both neutrophils in addition to mononuclear cells. Interleukin-17 was detectable in airway lavages from explanted cystic fibrosis lungs. Treatment of epithelial cultures with interleukin-17 increased production of interleukin-8, interleukin-6 and granulocyte macrophage colony-stimulating factor. In conclusion, immunoreactive interleukin-17 is raised in the lower airway of people with cystic fibrosis and localises to both neutrophils and mononuclear cells. Interleukin-17 increases production of pro-neutrophilic mediators by cystic fibrosis epithelial cells, suggesting potential for a positive feedback element in airway inflammation. |
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| Publisher | | European Respiratory Society |
| URL | | http://dx.doi.org/10.1183/09031936.00067110 |
| DOI | | 10.1183/09031936.00067110 |
| PubMed id | | 21109552 |
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