Detecting mismatch repair defects in myeloma

  1. Lookup NU author(s)
  2. Dr Mark Velangi
  3. Elizabeth Matheson
  4. Professor Andy Hall
  5. Dr Julie Irving
Author(s)Velangi M, Matheson E, Hall AG, Irving JAE
Editor(s)Brown RD; Ho PJ
Publication type Book Chapter
Book TitleMultiple Myeloma
Series TitleMethods in Molecular Medicine
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Defects in the mismatch repair system are associated with a microsatellite unstable phenotype. In this chapter, we describe the preparation of purified plasma cells using CD138 magnetic microbeads as a source of tumor DNA. We also describe a robust, sensitive method for comparing microsatellite repeat units of tumor to constitutive DNA using polymerase chain reaction and laser scanning of fluorescently labeled amplicons in an automated sequencer in order to assess microsatellite instability in myeloma.
PublisherHumana Press, Inc.
Place PublishedTotowa, N.J
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