Detecting mismatch repair defects in myeloma
- Lookup NU author(s)
- Dr Mark Velangi
- Elizabeth Matheson
- Professor Andy Hall
- Dr Julie Irving
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| Author(s) | | Velangi M, Matheson E, Hall AG, Irving JAE |
| Editor(s) | | Brown RD; Ho PJ |
| Publication type | | Book Chapter |
| Book Title | | Multiple Myeloma |
| Series Title | | Methods in Molecular Medicine |
| Year | | 2005 |
| Volume | | 113 |
| Pages | | 269-77 |
| ISBN | | 9781588293923 |
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| Full text for this publication is not currently held within this repository. Alternative links are provided below where available. |
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| Defects in the mismatch repair system are associated with a microsatellite unstable phenotype. In this chapter, we describe the preparation of purified plasma cells using CD138 magnetic microbeads as a source of tumor DNA. We also describe a robust, sensitive method for comparing microsatellite repeat units of tumor to constitutive DNA using polymerase chain reaction and laser scanning of fluorescently labeled amplicons in an automated sequencer in order to assess microsatellite instability in myeloma. |
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| Publisher | | Humana Press, Inc. |
| Place Published | | Totowa, N.J |
| URL | | http://www.springer.com/medicine/internal/book/978-1-58829-392-3 |
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| Library holdings | | Search Newcastle University Library for this item |
