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Lookup NU author(s): Mizue Yamazaki, Professor Tony Clare
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Eicosanoids are oxygenated derivatives of C20 polyunsaturated fatty acids known to play key roles in many physiological events in both invertebrates and vertebrates. The eicosanoid generating capacity of cypris larvae of the barnacle, Balanus amphitrite, was examined using enzyme immunoassay and high-performance liquid chromatography. These larvae generated the lipoxygenase products, 12-hydroxyeicosapentaenoic acid (HEPE), 8-HEPE and 8,15-diHEPE, together with the cyclooxygenase products, prostaglandin (PG) E, PGF and thromboxane (TX) B. Indomethacin, a selective cyclooxygenase inhibitor, caused a dose-dependent inhibition of PGE generation by B. amphitrite larvae, while esculetin and nordihydroguaiaretic acid (lipoxygenase inhibitors) also strongly inhibited the generation of 8-HEPE, 12-HEPE and 8,15-diHEPE. PGE2, PGE3 and 16,16-dimethyl PGE2 caused a dose-dependent inhibition of settlement of B. amphitrite larvae while indomethacin (25–100 [mu]M) stimulated this process. Lipoxygenase products (8-HEPE, 12-HEPE and 8,15-diHEPE) as well as esculetin and nordihydroguaiaretic acid (10–100 [mu]M) had no effect on the attachment of larvae.
Author(s): Knight J, Rowley AF, Yamazaki M, Clare AS
Publication type: Article
Publication status: Published
Journal: Journal of the Marine Biological Association of the UK
Year: 2000
Volume: 80
Issue: 1
Pages: 113-117
ISSN (print): 0025-3154
ISSN (electronic): 1469-7769
Publisher: Cambridge University Press
URL: http://dx.doi.org/10.1017/S0025315499001629
DOI: 10.1017/S0025315499001629
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