Electrochemical Oxidation of Glucose Using Mutant Glucose Oxidase from Directed Protein Evolution for Biosensor and Biofuel Cell Applications
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- Dr Eileen Yu
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| Author(s) | | Yu EH, Prodanovic R, Guven G, Ostafe R, Schwaneberg U |
| Publication type | | Article |
| Journal | | Applied Biochemistry and Biotechnology |
| Year | | 2011 |
| Volume | | 165 |
| Issue | | 7-8 |
| Pages | | 1448-1457 |
| ISSN (print) | | 0273-2289 |
| ISSN (electronic) | | 1559-0291 |
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| Full text for this publication is not currently held within this repository. Alternative links are provided below where available. |
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| In this study, electrochemical characterisation of glucose oxidation has been carried out in solution and using enzyme polymer electrodes prepared by mutant glucose oxidase (B11-GOx) obtained from directed protein evolution and wild type enzymes. Higher glucose oxidation currents were obtained from B11-GOx both in solution and polymer electrodes compared to wt-GOx. This demonstrates an improved electrocatalytic activity towards electrochemical oxidation of glucose from the mutant enzyme. The enzyme electrode with B11-GOx also showed a faster electron transfer indicating a better electronic interaction with the polymer mediator. These encouraging results have shown a promising application of enzymes developed by directed evolution tailored for the applications of biosensors and biofuel cells. |
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| Publisher | | Humana Press, Inc. |
| URL | | http://dx.doi.org/10.1007/s12010-011-9366-0 |
| DOI | | 10.1007/s12010-011-9366-0 |
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