Electrochemical Oxidation of Glucose Using Mutant Glucose Oxidase from Directed Protein Evolution for Biosensor and Biofuel Cell Applications

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  2. Dr Eileen Yu
Author(s)Yu EH, Prodanovic R, Guven G, Ostafe R, Schwaneberg U
Publication type Article
JournalApplied Biochemistry and Biotechnology
Year2011
Volume165
Issue7-8
Pages1448-1457
ISSN (print)0273-2289
ISSN (electronic)1559-0291
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In this study, electrochemical characterisation of glucose oxidation has been carried out in solution and using enzyme polymer electrodes prepared by mutant glucose oxidase (B11-GOx) obtained from directed protein evolution and wild type enzymes. Higher glucose oxidation currents were obtained from B11-GOx both in solution and polymer electrodes compared to wt-GOx. This demonstrates an improved electrocatalytic activity towards electrochemical oxidation of glucose from the mutant enzyme. The enzyme electrode with B11-GOx also showed a faster electron transfer indicating a better electronic interaction with the polymer mediator. These encouraging results have shown a promising application of enzymes developed by directed evolution tailored for the applications of biosensors and biofuel cells.
PublisherHumana Press, Inc.
URLhttp://dx.doi.org/10.1007/s12010-011-9366-0
DOI10.1007/s12010-011-9366-0
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