Type B T cells specific for an arthritogenic epitope of proteoglycan aggrecan recognize ligands released from degrading cartilage

  1. Lookup NU author(s)
  2. Dr Jane Falconer
  3. Dr Rahul Mahida
  4. Divya Venkatesh
  5. Professor John Robinson
Author(s)Falconer J, Mahida R, Venkatesh D, Pearson J, Robinson JH
Publication type Article
JournalJournal of Immunology
Year2012
Volume
Issue
Pages
ISSN (print)0022-1767
ISSN (electronic)1550-6606
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It has been proposed that peptide epitopes bind to MHC class II molecules to form distinct structural conformers of the same MHC-II-peptide complex termed type A and type B, and that the two conformers of the same peptide-MHC-II complex are recognized by distinct CD4 T cells, termed type A and type B T cells. As type B T cells that recognise exogenous self peptides have been shown to escape negative selection during thymic development they have the potential to contribute to the pathogenesis of autoimmunity. 0 0 1 211 1203 Newcastle University 10 2 1412 14.0 Normal 0 false false false EN-GB JA X-NONE We generated and characterised mouse CD4 T cells specific for an arthritogenic epitope of the candidate joint autoantigen proteoglycan aggrecan. Cloned T cell hybridomas specific for a synthetic peptide containing the aggrecan epitope divided into two groups based on their ability to recognise intact aggrecan and fine mapping demonstrated that both types of T cell recognised the same core epitope. The data is consistent with the generation of aggrecan-specific type A and type B T cells. Type B T cells were activated by supernatants released from degrading cartilage indicating the presence of antigenic fragments of aggrecan. We suggest that type B T cells could play a role in the pathogenesis of proteoglycan-induced arthritis in mice, a model for Rheumatoid Arthritis in man, by recognising extracellular peptides or protein fragments of joint autoantigens released by inflamed cartilage.
PublisherAmerican Association of Immunologists
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