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Comparison of Laboratory Wastewater Treatment Systems with Settlers: Affect of cycloheximide and recirculation ratio
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Dr Mariana Ferdes
Professor David Graham
Professor Vasile Lavric
Stoenica M, Buzatu P, Ferdes M, Ofiteri ID, Graham DW, Lavric V
Revista de Chimie
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The influence of cycloheximide and recirculation ratio upon system performance and microbial viability was studied in two continuous lab-scale wastewater treatment systems. Each system consists of an aerobic bioreactor, a settler, a sludge recycling line, and a sludge wastage line (purge). The addition of cycloheximide, which is a eukaryotic inhibitor that primarily impacts microbial predators, significantly (p < 0.05) reduced chemical oxygen demand (COD) removal efficiencies in the systems, but had no affect on ammonia removal (i.e., biological nitrification). However, complete nitrification was more apparent in the reactor system with the lower sludge recirculation ratio, especially when cycloheximide was being added. Although the temporal dynamics of living versus dead bacterial cell densities in the reactors did not differ as a function of cycloheximide addition or recirculation ratio, the data show that a stable and diverse predator guild creates a more stable treatment community and more consistent treatment performance.
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