Probing the skin permeation of fish oil/EPA and ketoprofen-3. Effects on epidermal COX-2 and LOX
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- Dr Zoe Davison
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| Author(s) | | Thomas CP, Davison Z, Heard CM |
| Publication type | | Article |
| Journal | | Prostaglandins, Leukotrienes and Essential Fatty Acids |
| Year | | 2007 |
| Volume | | 76 |
| Issue | | 6 |
| Pages | | 357-362 |
| ISSN (print) | | 0952-3278 |
| ISSN (electronic) | | 1532-2823 |
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| Full text for this publication is not currently held within this repository. Alternative links are provided below where available. |
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| This work employed immunocytochemistry (ICC) techniques to study the effect of topically applied fish oil and ketoprofen on cyclooxygenase (COX-2) and lipoxygenase (LOX) within freshly excised porcine ear skin. Maintained in Hanks buffer immediately post excision, full thickness membranes were mounted in Franz diffusion cells and dosed with 1 ml of individual formulations containing ketoprofen, fish oil or both. At different timepoints, the diffused areas were recovered and relative activities of COX-2 and LOX determined. It was found that the fish oil formulation qualitatively inhibited the expression of both COX-2 and LOX enzymes. As expected, ketoprofen had no effect upon LOX expression but a significant decrease on COX-2 expression was observed. The formulation containing both fish oil and ketoprofen proved to be the most effective at inhibiting the expression of both COX-2 and LOX. Considered together with data from earlier papers, a mechanism of EPA permeation enhancement by ketoprofen may be elucidated and also show the ability of such a formulation to inhibit these enzymes and thus indicate the efficacy of such a formulation. |
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| Publisher | | Churchill Livingstone |
| URL | | http://dx.doi.org/10.1016/j.plefa.2007.05.002 |
| DOI | | 10.1016/j.plefa.2007.05.002 |
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