High-throughput analysis of mouse embryos by magnetic resonance imaging

  1. Lookup NU author(s)
  2. Dr Simon Bamforth
Author(s)Bamforth SD, Schneider JE, Bhattacharya S
Publication type Article
JournalCold Spring Harbor Protocols
Year2012
Volume2012
Issue1
Pages93-101
ISSN (print)1940-3402
ISSN (electronic)1559-6095
Full text for this publication is not currently held within this repository. Alternative links are provided below where available.
Genetic studies in the mouse are crucial for uncovering new genes and signaling pathways associated with development. The identification of murine models with developmental malformations in high-throughput mutagenesis screens is made difficult because, after mid-embryogenesis, the embryo is opaque. Traditional phenotyping methods such as histological sectioning are labor intensive and destructive. We have developed and optimized a novel method for high-throughput multiembryo magnetic resonance imaging (MRI). Here we present our method for processing 32 mouse embryos for analysis by MRI. We describe the MR system, imaging software, and the reconstruction of two-dimensional (2D) and three-dimensional (3D) images. We also discuss the applications of this technique, highlight its advantages, and point out some disadvantages. Using this approach, we can identify developmental malformations in mutant embryos at high spatial resolution (voxel size 25.4 × 25.4 × 24.4 µm). This technique can be easily used for mouse mutagenesis screens and thus provides an important tool for identifying new mouse models for human diseases.
PublisherCold Spring Harbor Laboratory Press
URLhttp://dx.doi.org/10.1101/pdb.prot067538
DOI10.1101/pdb.prot067538
Actions    Link to this publication