Enzyme activity demonstrates multiple pathways of innate immunity in Indo-Pacific anthozoans

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  2. Caroline Palmer
  3. Professor John Bythell
Author(s)Palmer CV, Bythell JC, Willis BL
Publication type Article
JournalProceedings of the Royal Society B: Biological Sciences
Year2012
Volume279
Issue1743
Pages3879-3887
ISSN (print)0962-8452
ISSN (electronic)1471-2954
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Coral reefs are threatened by increasing levels of coral disease and the functional loss of obligate algal symbionts (bleaching). Levels of immunity relate directly to susceptibility to these threats; however, our understanding of fundamental aspects of coral immunology is lacking. We show that three melanin-synthesis pathway components (mono-phenoloxidase, ortho-diphenoloxidase (tyrosinase-type pathway) and para-diphenoloxidase (laccase-type pathway)) are present in both their active (phenoloxidase, PO) and inactive (prophenoloxidase, PPO) forms across a diverse range of 22 species of healthy Indo-Pacific anthozoans. We also demonstrate transglutaminase activity of the coagulation cascade for, to our knowledge, the first time in a coral. Melanin-synthesis enzyme activities varied among taxa, although they were generally lowest in the coral family Acroporidae and highest in the Poritidae and Oculinidae. Inactive tyrosinase-type activity (PPO) and active laccase-type activity (PO) correlate with taxonomic patterns in disease resistance, whereas the converse pattern in activity levels correlates with bleaching resistance. Overall, we demonstrate the presence of several melanin-synthesis pathways in Indo-Pacific corals, co-regulation among some pathway components, and highlight their potential roles in coral health.
PublisherThe Royal Society Publishing
URLhttp://dx.doi.org/10.1098/rspb.2011.2487
DOI10.1098/rspb.2011.2487
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