About Open Access
Expression, crystallization, preliminary X-ray data analysis of NT-ALS9, a fungal adhesin from
Lookup NU author(s)
Dr Paula Salgado
Salgado PS, Yan R, Rowan F, Cota E
Acta Crystallographica. Section F: Structural Biology and Crystallization Communications
Full text for this publication is not currently held within this repository. Alternative links are provided below where available.
Candida albicans is a common human fungal commensal that can also cause a range of infections from skin/mucosal `thrush' to severe systemic candidiasis. Adherence to host cells is one of the key determinants of Candida pathogenesis. The Als family of surface proteins has been implicated in adhesion of C. albicans, yet limited information has been published on the structure and mechanism of these fungal adhesins. The N-terminal region of these proteins has been shown to possess adhesive properties, making it a possible target for new therapeutic strategies. Recombinant NT-Als9-2 from C. albicans (residues 18-329) was overexpressed in Escherichia coli, purified and crystallized. Diffraction data were collected to 2.0 Å resolution. The crystals belonged to space group P212121, with unit-cell parameters a = 34.73, b = 68.71, c = 120.03 Å, [alpha] = [beta] = [gamma] = 90° and one molecule in the asymmetric unit. Platinum-derivatized crystals belonged to the same space group, with similar unit-cell parameters, although they were not completely isomorphous.
Wiley-Blackwell Publishing, Inc.
Newcastle University Library, NE2 4HQ, United Kingdom. Tel: 0044 (191) 222 7657
©2014 Newcastle University Library