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Nonlinear regression analysis (NLR) is applied to quantify the dynamic response of non-photochemical fluorescence quenching (NPQ) of Trifolium repens cv. Regal upon dark to light transition. Commonly, only steady-state levels of NPQ are evaluated, ignoring transient kinetics. Experimental NPQ kinetics are fitted best with a sum of two functions: a sigmoidal Hill function plus a transient logarithmic normal function. It is shown that not only steady-state level of NPQ, but also the speed at which steady state is reached, increased with light intensity. The question is raised which biological processes cause the induction of the components of NPQ kinetics. The NPQ kinetics are found to resemble the kinetics of antheraxanthin and zeaxanthin formation during a dark to light transition. Furthermore, both molecules are known to induce NPQ. The hypothesis is put forward that a transient phase of NPQ (0-2 min after transition) is dependent upon concentrations of antheraxanthin, whereas the saturating phase corresponds with the production of zeaxanthin. A mathematical model, based on the presented hypothesis, predicts the effect of increasing light intensity on concentrations of antheraxanthin and zeaxanthin which correspond with experimental results. Implications of the hypothesis are discussed as well as the role of NLR in evaluating chlorophyll a fluorescence kinetics.
Author(s): D'Haese D, Vandermeiren K, Caubergs RJ, Guisez Y, De Temmerman L, Horemans N
Publication type: Article
Publication status: Published
Journal: Journal of Theoretical Biology
Year: 2004
Volume: 227
Issue: 2
Pages: 175-186
ISSN (print): 0022-5193
ISSN (electronic): 1095-8541
Publisher: Academic Press
URL: http://dx.doi.org/10.1016/j.jtbi.2003.10.011
DOI: 10.1016/j.jtbi.2003.10.011
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