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Lookup NU author(s): Dr SarahJayne Boulton, Professor Mark Birch-MachinORCiD
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
Melanogenesis is a highly conserved process of cytophotoprotection from UV radiation present in many species. Although both mitochondrial function and UV radiation insults are well-documented promoters of increased cellular stress, their individual molecular relationships with skin pigmentation have not been clearly resolved. This study provides evidence for a direct relationship between cellular melanin content, superoxide flux, and mitochondrial function at complex II. Direct and significant correlation between increased pigmentation and complex II turnover was observed in genetically different melanoma cell lines of varied basal pigmentation states (P < 0.01). The same trend was also observed when comparing genetically identical cell cultures with increasing levels of induced pigmentation (P < 0.005). The observation of increased steady-state levels of the catalytic complex II succinate dehydrogenase subunit A alongside hyperpigmentation suggested coregulation of activity and pigment production (P < 0.01). The study also presents novel evidence for a relationship between hyperpigmentation and increased superoxide-generating capacity at complex II. By amperometrically monitoring superoxide flux from differently pigmented FM55 melanocytes and their isolated mitochondria, a dynamic and responsive relationship between pigmentation, complex II function, and intracellular superoxide generation was observed (P < 0.005). The data support hyperpigmentation as a protective antioxidant mechanism in response to complex II-mediated reactive oxygen species generation.—Boulton, S. J., Birch-Machin, M. A. Impact of hyperpigmentationonsuperoxidefluxandmelanoma cell metabolism at mitochondrial complex II.
Author(s): Boulton SJ, Birch-Machin MA
Publication type: Article
Publication status: Published
Journal: The FASEB Journal
Year: 2015
Volume: 29
Issue: 1
Pages: 346-353
Print publication date: 01/01/2015
Online publication date: 28/10/2014
Acceptance date: 15/09/2014
Date deposited: 18/12/2014
ISSN (print): 0892-6638
ISSN (electronic): 1530-6860
Publisher: Federation of American Societies for Experimental Biology
URL: http://dx.doi.org/10.1096/fj.14-261982
DOI: 10.1096/fj.14-261982
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