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Nocardiopsis algeriensis sp. nov., an alkalitolerant actinomycete isolated from Saharan soil

Lookup NU author(s): Professor Hans-Peter Klenk

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Abstract

An alkalitolerant actinomycete strain, designated B32^T, was isolated from a Saharan soil sample collected from Adrar province (South of Algeria), and then investigated using a polyphasic taxonomic approach. The strain was observed to produce short chains of spores on the dichotomous branched aerial mycelium and formed a fragmented substrate mycelium. The optimum NaCl concentration for growth was found to be 0–5 % (w/v) and the optimum growth temperature and pH were found to be 25–35 °C and 7.0–10.0 °C, respectively. The diagnostic diamino acid in the cell-wall peptidoglycan was identified as meso-diaminopimelic acid. The predominant menaquinones of strain B32^T were identified as MK-10 (H₄) and MK-11 (H₄). The major fatty acids were found to be iso-C_16:0 and anteiso-C_15:0. The diagnostic phospholipids detected were phosphatidylcholine, phosphatidylmethylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The chemotaxonomic properties of strain B32^T are consistent with those shared by members of the genus Nocardiopsis. 16S rRNA gene sequence analysis indicated that strain B32^T is most closely related to Nocardiopsis alba DSM 43377^T (98.7 %), Nocardiopsis lucentensis DSM 44048^T (98.6 %), Nocardiopsis aegyptia DSM 44442^T (98.6 %), Nocardiopsis sinuspersici HM6^T (98.6 %) and Nocardiopsis arvandica HM7^T (98.5 %). However, the DNA–DNA relatedness values between strain B32^T and the closely related type strains were 17.9, 14.6, 31.1, 27.1 and 14.1 %, respectively. Based on the combined genotypic and phenotypic evidence, it is proposed that strain B32^T should be classified as representative of a novel species, for which the name Nocardiopsis algeriensis sp. nov. is proposed. The type strain is B32^T (=DSM 45462^T = CECT 8712^T).

Publication metadata

Author(s): Bouras N, Meklat A, Zitouni A, Mathieu F, Schumann P, Spröer C, Sabaou N, Klenk HP

Publication type: Article

Publication status: Published

Journal: Antonie van Leeuwenhoek

Year: 2015

Volume: 107

Issue: 2

Pages: 313-320

Print publication date: 01/02/2015

Online publication date: 08/11/2014

Acceptance date: 03/11/2014

ISSN (print): 0003-6072

ISSN (electronic): 1572-9699

Publisher: Springer

URL: http://dx.doi.org/10.1007/s10482-014-0329-7

DOI: 10.1007/s10482-014-0329-7

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