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Actinopolyspora biskrensis sp. nov., a Novel Halophilic Actinomycete Isolated from Northern Sahara

Lookup NU author(s): Professor Hans-Peter Klenk

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Abstract

A novel halophilic, filamentous actinomycete, designated H254^T, was isolated from a Saharan soil sample collected from Biskra (Northern Sahara), and subjected to a polyphasic taxonomic characterization. The strain is Gram-positive, aerobic, and halophilic, and the optimum NaCl concentration for growth is 15–20 % (w/v). The cell-wall hydrolysate contained meso-diaminopimelic acid, and the diagnostic whole-cell sugars were arabinose and galactose. The diagnostic phospholipid detected was phosphatidylcholine, and MK-9(H₄) was the predominant menaquinone. The major fatty acid profiles were anteiso-C_17:0 (32.8 %), C_15:0 (28 %), and iso-C_17:0 (12.3 %). Comparative analysis of the 16S rRNA gene sequences revealed that the strain H254^T formed a well-separated sub-branch within the radiation of the genus Actinopolyspora, and the microorganism was most closely related to Actinopolyspora saharensis DSM 45459^T (99.2 %), Actinopolyspora halophila DSM 43834^T (99.1 %), and Actinopolyspora algeriensis DSM 45476^T (99.0 %). Nevertheless, the strain had relatively lower mean values for DNA–DNA relatedness with the above strains (57.2, 68.4, and 45.6 %, respectively). Based on phenotypic features and phylogenetic position, we propose that strain H254^T represents a novel species of the genus Actinopolyspora, for which the name Actinopolyspora biskrensis sp. nov. is proposed. The type strain of A. biskrensis is strain H254^T (=DSM 46684^T =CECT 8576^T).

Publication metadata

Author(s): Saker R, Bouras N, Meklat A, Zitouni A, Schumann P, Spröer C, Klenk HP, Sabaou N

Publication type: Article

Publication status: Published

Journal: Current Microbiology

Year: 2015

Volume: 70

Issue: 3

Pages: 423-428

Print publication date: 01/03/2015

Online publication date: 28/11/2014

Acceptance date: 29/10/2014

ISSN (print): 0343-8651

ISSN (electronic): 1432-0991

Publisher: Springer

URL: http://dx.doi.org/10.1007/s00284-014-0740-3

DOI: 10.1007/s00284-014-0740-3

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