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CD1c+ blood dendritic cells have Langerhans cell potential

Lookup NU author(s): Dr Paul Milne, Dr Venetia BigleyORCiD, Dr Merry Gunawan, Professor Muzlifah Haniffa, Professor Matthew CollinORCiD

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Abstract

Langerhans cells (LCs) are self-renewing in the steady state but repopulated by myeloid precursors after injury. Human monocytes give rise to langerin-positive cells in vitro, suggesting a potential precursor role. However, differentiation experiments with human lineage-negative cells and CD34(+) progenitors suggest that there is an alternative monocyte-independent pathway of LC differentiation. Recent data in mice also show long-term repopulation of the LC compartment with alternative myeloid precursors. Here we show that, although monocytes are able to express langerin, when cultured with soluble ligands granulocyte macrophage colony-stimulating factor (GM-CSF), transforming growth factor beta (TGF beta), and bone morphogenetic protein 7 (BMP7), CD1c(+) dendritic cells (DCs) become much more LC-like with high langerin, Birbeck granules, EpCAM, and E-cadherin expression under the same conditions. These data highlight a new potential precursor function of CD1c(+) DCs and demonstrate an alternative pathway of LC differentiation that may have relevance in vivo.


Publication metadata

Author(s): Milne P, Bigley V, Gunawan M, Haniffa M, Collin M

Publication type: Article

Publication status: Published

Journal: Blood

Year: 2015

Volume: 125

Issue: 3

Pages: 470-473

Print publication date: 15/01/2015

Online publication date: 28/10/2014

Acceptance date: 14/10/2014

ISSN (print): 0006-4971

ISSN (electronic): 1528-0020

Publisher: American Society of Hematology

URL: http://dx.doi.org/10.1182/blood-2014-08-593582

DOI: 10.1182/blood-2014-08-593582


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Funding

Funder referenceFunder name
Histiocytosis Association
Histiocytosis Research Trust
1105891Bright Red (registered charity)
WT101155/Z/13/ZWellcome Trust
WT088555MAWellcome Trust

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