A self-assembling fluorescent dipeptide conjugate for cell labelling

  1. Lookup NU author(s)
  2. Dr Ricardo Martins Gouveia
  3. Professor Che Connon
Author(s)Kirkham S, Hamley IW, Smith AM, Gouveia RM, Connon CJ, Reza M, Ruokolainen J
Publication type Article
JournalColloids and Surfaces B: Biointerfaces
Year2016
Volume137
Issue
Pages104-108
ISSN (print)0927-7765
ISSN (electronic)1873-4367
Full text is available for this publication:
Derivatives of fluorophore FITC (fluorescein isothiocyanate) are widely used in bioassays to label proteins and cells. An N-terminal leucine dipeptide is attached to FITC, and we show that this simple conjugate molecule is cytocompatible and is uptaken by cells (human dermal and corneal fibroblasts) in contrast to FITC itself. Co-localisation shows that FITC-LL segregates in peri-nuclear and intracellular vesicle regions. Above a critical aggregation concentration, the conjugate is shown to self-assemble into beta-sheet nanostructures comprising molecular bilayers.
PublisherElsevier
URLhttp://dx.doi.org/10.1016/j.colsurfb.2015.04.062
DOI10.1016/j.colsurfb.2015.04.062
PubMed id25990811
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