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A self-assembling fluorescent dipeptide conjugate for cell labelling

Lookup NU author(s): Dr Ricardo Martins GouveiaORCiD, Professor Che ConnonORCiD

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Abstract

Derivatives of fluorophore FITC (fluorescein isothiocyanate) are widely used in bioassays to label proteins and cells. An N-terminal leucine dipeptide is attached to FITC, and we show that this simple conjugate molecule is cytocompatible and is uptaken by cells (human dermal and corneal fibroblasts) in contrast to FITC itself. Co-localisation shows that FITC-LL segregates in peri-nuclear and intracellular vesicle regions. Above a critical aggregation concentration, the conjugate is shown to self-assemble into beta-sheet nanostructures comprising molecular bilayers.

Publication metadata

Author(s): Kirkham S, Hamley IW, Smith AM, Gouveia RM, Connon CJ, Reza M, Ruokolainen J

Publication type: Article

Publication status: Published

Journal: Colloids and Surfaces B: Biointerfaces

Year: 2016

Volume: 137

Pages: 104-108

Print publication date: 01/01/2016

Online publication date: 07/05/2015

Acceptance date: 28/04/2015

Date deposited: 17/06/2015

ISSN (print): 0927-7765

ISSN (electronic): 1873-4367

Publisher: Elsevier

URL: http://dx.doi.org/10.1016/j.colsurfb.2015.04.062

DOI: 10.1016/j.colsurfb.2015.04.062

PubMed id: 25990811

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