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Lookup NU author(s): Dr Artur Rogowski, Carl Morland, Dr Alan Cartmell, Dr Lucy Crouch, Dr Aurore Labourel, Professor William WillatsORCiD, Emeritus Professor Harry Gilbert
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Type A non-catalytic carbohydrate-binding modules (CBMs), exemplified by CtCBM3a(cipA), are widely believed to specifically target crystalline cellulose through entropic forces. Here we have tested the hypothesis that type A CBMs can also bind to xyloglucan (XG), a soluble beta-1,4-glucan containing alpha-1,6-xylose side chains. CtCBM3a(cipA) bound to xyloglucan in cell walls and arrayed on solid surfaces. Xyloglucan and cellulose were shown to bind to the same planar surface on CBM3a(cipA). A range of type A CBMs from different families were shown to bind to xyloglucan in solution with ligand binding driven by enthalpic changes. The nature of CBM-polysaccharide interactions is discussed. (C) 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Author(s): Hernandez-Gomez MC, Rydahl MG, Rogowski A, Morland C, Cartmell A, Crouch L, Labourel A, Fontes CMGA, Willats WGT, Gilbert HJ, Knox JP
Publication type: Article
Publication status: Published
Journal: FEBS Letters
Year: 2015
Volume: 589
Issue: 18
Pages: 2297-2303
Print publication date: 19/08/2015
Online publication date: 18/07/2015
Acceptance date: 07/07/2015
ISSN (print): 0014-5793
ISSN (electronic): 1873-3468
Publisher: Elsevier
URL: http://dx.doi.org/10.1016/j.febslet.2015.07.009
DOI: 10.1016/j.febslet.2015.07.009
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