Alginate-Encapsulation for the Improved Hypothermic Preservation of Human Adipose-Derived Stem Cells

  1. Lookup NU author(s)
  2. Dr Stephen Swioklo
  3. Dr Andrei Constantinescu
  4. Professor Che Connon
Author(s)Swioklo S, Constantinescu A, Connon CJ
Publication type Article
JournalStem Cells Translational Medicine
Year2016
Volume5
Issue3
Pages339-349
ISSN (print)2157-6564
ISSN (electronic)2157-6580
Full text is available for this publication:
Despite considerable progress within the cell therapy industry, unmet bioprocessing and logistical challenges associated with the storage and distribution of cells between sites of manufacture and the clinic exist. We examined whether hypothermic (4°C–23°C) preservation of human adipose-derived stem cells could be improved through their encapsulation in 1.2% calcium alginate. Alginate encapsulation improved the recovery of viable cells after 72 hours of storage. Viable cell recovery was highly temperature dependent, with an optimum temperature of 15°C. At this temperature, alginate encapsulation preserved the ability for recovered cells to attach to tissue culture plastic on rewarming, further increasing its effect on total cell recovery. On attachment, the cells were phenotypically normal, displayed normal growth kinetics, and maintained their capacity for trilineage differentiation. The number of cells encapsulated (up to 2 Å~106 cells per milliliter) did not affect viable cell recovery nor did storage of encapsulated cells in a xenofree, serum-free, current Good Manufacturing Practice-grade medium. We present a simple, low-cost system capable of enhancing the preservation of human adipose-derived stem cells stored at hypothermic temperatures, while maintaining their normal function. The storage of cells in this manner has great potential for extending the time windows for quality assurance and efficacy testing, distribution between the sites of manufacture and the clinic, and reducing the wastage associated with the limited shelf life of cells stored in their liquid state.
PublisherAlphaMed Press, Inc.
URLhttp://dx.doi.org/10.5966/sctm.2015-0131
DOI10.5966/sctm.2015-0131
PubMed id26826163
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