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A region encompassing the FERM domain of Jak1 is necessary for binding to the cytokine receptor gp130

Lookup NU author(s): Professor Catharien Hilkens

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Abstract

The terminal portion of the Janus kinases (Jaks) contains a divergent FERM (Four-point-one, Ezrin, Radixin, Moesin) homology domain comprising 19 conserved hydrophobic regions. To determine the role of this domain in governing recruitment of Jak1, but not Jak3, to the gp130 subunit of the interleukin-6 family of cytokine receptors, the interaction of three Jak1/Jak3 chimeras with gp130 was investigated. Chimeras 1, 2 and 3 (Jak1 FERM regions 1-19, 1-18 and 1-8/Jak3, respectively) were all enzymically active. Chimeras 1 and 2 interacted with the cytoplasmic domain of gp130, although less efficiently than Jak1. Only chimera 2, however, restored gp130 signalling in Jak1-negative cells. The data are consistent with recruitment of Jak1 to gp130 through the Jak1 FERM domain, but also emphasise the likely requirement for precise Jak/receptor orientation to sustain function.

Publication metadata

Author(s): Hilkens CM, Is'harc H, Lillemeier BF, Strobl B, Bates PA, Behrmann I, Kerr IM

Publication type: Article

Publication status: Published

Journal: FEBS Letters

Year: 2001

Volume: 505

Issue: 1

Pages: 87-91

Print publication date: 14/08/2001

ISSN (print): 0014-5793

ISSN (electronic): 1873-3468

Publisher: Elsevier BV

URL: http://dx.doi.org/10.1016/S0014-5793(01)02783-1

DOI: 10.1016/S0014-5793(01)02783-1

Notes: 0014-5793 Journal Article

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