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Acidic chitinase primes the protective immune response to gastrointestinal nematodes

Lookup NU author(s): Dr Lee Borthwick

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This is the authors' accepted manuscript of an article that has been published in its final definitive form by Nature Publishing Group, 2016.

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Abstract

Acidic mammalian chitinase (AMCase) is known to be induced by allergens and helminths, yet its role in immunity is unclear. Using AMCase-deficient mice, we show that AMCase deficiency reduced the number of group 2 innate lymphoid cells during allergen challenge but was not required for establishment of type 2 inflammation in the lung in response to allergens or helminths. In contrast, AMCase-deficient mice showed a profound defect in type 2 immunity following infection with the chitin-containing gastrointestinal nematodes Nippostrongylus brasiliensis and Heligmosomoides polygyrus bakeri. The impaired immunity was associated with reduced mucus production and decreased intestinal expression of the signature type 2 response genes Il13, Chil3, Retnlb, and Clca1. CD103(+) dendritic cells, which regulate T cell homing, were also reduced in mesenteric lymph nodes of infected AMCase-deficient mice. Thus, AMCase functions as a critical initiator of protective type 2 responses to intestinal nematodes but is largely dispensable for allergic responses in the lung.


Publication metadata

Author(s): Vannella KM, Ramalingam TR, Hart KM, Prado RD, Sciurba J, Barron L, Borthwick LA, Smith AD, Mentink-Kane M, White S, Thompson RW, Cheever AW, Bock K, Moore I, Fitz LJ, Urban JF, Wynn TA

Publication type: Article

Publication status: Published

Journal: Nature Immunology

Year: 2016

Volume: 17

Issue: 5

Pages: 538-544

Print publication date: 01/05/2016

Online publication date: 04/04/2016

Acceptance date: 18/02/2016

Date deposited: 16/06/2016

ISSN (print): 1529-2908

ISSN (electronic): 1529-2916

Publisher: Nature Publishing Group

URL: http://dx.doi.org/10.1038/ni.3417

DOI: 10.1038/ni.3417


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