Impact of collagen/heparin multi-layers for regulating bone cellular functions

  1. Lookup NU author(s)
  2. Dr Ana Ferreira-Duarte
  3. Dr Piergiorgio Gentile
  4. Dr Ria Toumpaniari
  5. Dr Mark Birch
Author(s)Ferreira AM, Gentile P, Toumpaniari S, Ciardelli G, Birch MA
Publication type Article
JournalACS Applied Materials & Interfaces
ISSN (print)1944-8244
ISSN (electronic)1944-8252
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Bone cell interaction with extracellular matrix (ECM) microenvironment is of critical importance when engineering surface interfaces for bone regeneration. In this work layer-by-layer films of type I collagen (coll), the major constituent of bone ECM, and heparin (hep), a glycosaminoglycan, were assembled on poly(L-lactide acid) (PLLA) substrates to evaluate the impact of the biomacromolecular coating on cell activity. The surface modification of PLLA demonstrated that the hep/coll multi-layer is stable after 10 bilayers (confirmed by contact angle, infrared spectroscopy and morphological analysis). This simple approach provided novel information on the effect of heparin on type I collagen hierarchical organisation and subsequent cell response of osteoblast-like (MC3T3-E1) and human bone marrow-derived mesenchymal stem cells (hMSCs). Interestingly, the number of deposited heparin layers (1 or 10) appeared to play an important role in the self-assembly of collagen into fibrils, stabilising the fibrous collagen layer, and potentially impacting on hMSCs activity.
PublisherAmerican Chemical Society
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