Non-covalently crosslinked chitosan nanofibrous mats prepared by electrospinning as substrates for soft tissue regeneration

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  2. Dr Piergiorgio Gentile
Author(s)Tonda-Turo C, Ruini F, Ramella M, Boccafoschi F, Gentile P, Gioffredi E, Falvo D'Urso Labate G
Publication type Article
JournalCarbohydrate Polymers
IssueePub ahead of Print
ISSN (print)0144-8617
ISSN (electronic)1879-1344
Full text is available for this publication:
itosan (CS) membranes obtained by electrospinning are potentially ideal substrates for soft tissue engineering as they combine the excellent biological properties of CS with the extracellular matrix (ECM)-like structure of nanofibrous mats. However, the high amount of acid solvents required to spun CS solutions interferes with the biocompatibility of CS fibres. To overcome this limitation, a novel CS based solutions were investigated in this work. Low amount of acidic acid (0.5 M) was used and dibasic sodium phosphate (DSP) was introduced as ionic crosslinker to improve nanofibres water stability and to neutralize the acidic pH of electrospun membranes after fibres soaking in biological fluids. Randomly oriented and aligned nanofibres (118 ± 16nm size) were obtained through electrospinning process (voltage of 30 kV, 30 μL/min flow rate and temperature of 39 °C) showing mechanical properties similar to those of soft tissues (Young Modulus lower than 40 MPa in dry condition) and water stability until 7 days. C2C12 myoblast cell line was cultured on CS fibres showing that the aligned architecture of substrate induces cell orientation that can enhance skeletal muscle regeneration.
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