Development of multi-substituted hydroxyapatite nanopowders as biomedical materials for bone tissue engineering applications

  1. Lookup NU author(s)
  2. Yanny Baba Ismail
  3. Dr Oana Bretcanu
  4. Professor Kenneth Dalgarno
Author(s)Baba Ismail YM, Wimpenny I, Bretcanu O, Dalgarno K, El Haj AJ
Publication type Article
JournalJournal of Biomedical Materials Research Part A
Pagesepub ahead of print
ISSN (print)1549-3296
ISSN (electronic)1552-4965
Full text is available for this publication:
Ionic substitutions have been proposed as a tool to control the functional behavior of synthetic hydroxyapatite (HA), particularly for Bone Tissue Engineering (BTE) applications. The effect of simultaneous substitution of different levels of carbonate (CO3) and silicon (Si) ions in the HA lattice was investigated. Furthermore, human bone marrow-derived mesenchymal stem cells (hMSCs) were cultured on multi-substituted HA (SiCHA) to determine if biomimetic chemical compositions were osteoconductive. Of the four different compositions investigates, SiCHA-1 (0.58wt% Si) and SiCHA-2 (0.45wt% Si) showed missing bands for CO3 and Si using FTIR analysis, indicating competition for occupation of the phosphate site in the HA lattice. 500°C was considered the most favourable calcination temperature as: (i) the powders produced possessed a similar amount of CO3 (2-8wt%) and Si (<1.0wt%) as present in native bone; and (ii) there was a minimal loss of CO3 and Si from the HA structure to the surroundings during calcination. Higher Si content in SiCHA-1 led to lower cell viability and at most hindered proliferation, but no toxicity effect occurred. While, lower Si content in SiCHA-2 showed the highest ALP/DNA ratio after 21 days culture with hMSCs, indicating that the powder may stimulate osteogenic behaviour to a greater extent than other powders.
PublisherJohn Wiley & Sons, Inc.
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