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MutPred mutational load analysis shows mildly deleterious mitochondrial DNA variants are not more prevalent in Alzheimer's patients, but may be under-represented in healthy older individuals

Lookup NU author(s): Dr Ilse Pienaar, Dr Joanna Elson

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND).


Abstract

© 2017 Elsevier B.V. and Mitochondria Research Society. Mitochondrial DNA (mtDNA) association studies have been conducted for over a decade using the haplogroup (lineage) association method, but this frequently produces conflicting results. Here we analyzed complete mtDNA sequence data of Alzheimer's disease (AD) patients and aged controls, from the United Kingdom (UK) and the United States (US), using a new "mutational load" method. We calculated a pathogenicity score for each of the non-synonymous substitutions of the mtDNA sequences to produce a "total mutational load" for each sequence, and compared the mutational loads of cases and controls. Using these mutational load measures, we found no evidence to support the cumulative role of mtDNA variants as a susceptibility factor in AD; that is, AD patients (UK and US cohorts) did not have higher "mutational loads" than controls. However, the US aged controls, who are significantly older than the UK ones, with many showing evidence of being healthy and having good cognition in old age, had significantly lower "mutational loads". This finding suggests that low mtDNA mutational load is more prevalent in healthy older people.


Publication metadata

Author(s): Pienaar IS, Howell N, Elson JL

Publication type: Article

Publication status: Published

Journal: Mitochondrion

Year: 2017

Volume: 34

Pages: 141-146

Print publication date: 01/05/2017

Online publication date: 07/04/2017

Acceptance date: 06/04/2017

Date deposited: 05/07/2017

ISSN (print): 1567-7249

ISSN (electronic): 1872-8278

Publisher: Elsevier B.V.

URL: http://doi.org/10.1016/j.mito.2017.04.002

DOI: 10.1016/j.mito.2017.04.002


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