The fate and lifespan of human monocyte subsets in steady state and systemic inflammation

  1. Lookup NU author(s)
  2. Amit Patel
  3. Dr Venetia Bigley
Author(s)Patel AA, Zhang Y, Fullerton JN, Boelen L, Rongvaux A, Maini AA, Bigley V, Flavell RA, Gilroy DW, Asquith B, Macallan D, Yona S
Publication type Article
JournalJournal of Experimental Medicine
Year2017
Volume
Issue
PagesEpub ahead of print
ISSN (print)0022-1007
ISSN (electronic)1540-9538
Full text is available for this publication:
In humans, the monocyte pool comprises three subsets (classical, intermediate, and nonclassical) that circulate in dynamic equilibrium. The kinetics underlying their generation, differentiation, and disappearance are critical to understanding both steady-state homeostasis and inflammatory responses. Here, using human in vivo deuterium labeling, we demonstrate that classical monocytes emerge first from marrow, after a postmitotic interval of 1.6 d, and circulate for a day. Subsequent labeling of intermediate and nonclassical monocytes is consistent with a model of sequential transition. Intermediate and nonclassical monocytes have longer circulating lifespans (∼4 and ∼7 d, respectively). In a human experimental endotoxemia model, a transient but profound monocytopenia was observed; restoration of circulating monocytes was achieved by the early release of classical monocytes from bone marrow. The sequence of repopulation recapitulated the order of maturation in healthy homeostasis. This developmental relationship between monocyte subsets was verified by fate mapping grafted human classical monocytes into humanized mice, which were able to differentiate sequentially into intermediate and nonclassical cells.
PublisherRockefeller University Press
URLhttps://doi.org/10.1084/jem.20170355
DOI10.1084/jem.20170355
PubMed id28606987
Actions    Link to this publication

Altmetrics provided by Altmetric

Share