About Open Access
Overexpression of sucrose phosphate synthase increases sucrose unloading in transformed tomato fruit
Lookup NU author(s)
Professor Christine Foyer
Nguyen-Quoc B, N'Tchobo H, Foyer CH, Yelle S
Journal of Experimental Botany
Full text for this publication is not currently held within this repository. Alternative links are provided below where available.
Sucrose unloading and sink activity were examined in tomato plants (Lycopersicon esculentum) overexpressing sucrose phosphate synthase (SPS; EC 126.96.36.199). Like the leaves, the fruit of the transformed tomato plants had elevated (2.4-fold) SPS activity. SPS over-expression in tomato fruit did not significantly change acid invertase, and only slightly reduced ADPglc ppase activity, but enhanced sucrose synthase activity by 27%. More importantly, the amount of sucrose unloaded into the fruit was considerably increased. Using [3H]- (fructosyl)-sucrose in in vitro unloading experiments with harvested 20-d-old fruit, 70% more sucrose was unloaded into the transformed fruits compared to the untransformed controls. Furthermore, the turnover of the sucrose unloaded into the fruit of transformed plants was 60% higher than that observed in the untransformed controls. Taken together, these results demonstrate that SPS overexpression increases the sink strength of transformed tomato fruit.
Oxford University Press
Newcastle University Library, NE2 4HQ, United Kingdom. Tel: 0044 (191) 222 7657
©2015 Newcastle University Library