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Overexpression of sucrose phosphate synthase increases sucrose unloading in transformed tomato fruit
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Professor Christine Foyer
Nguyen-Quoc B, N'Tchobo H, Foyer CH, Yelle S
Journal of Experimental Botany
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Sucrose unloading and sink activity were examined in tomato plants (Lycopersicon esculentum) overexpressing sucrose phosphate synthase (SPS; EC 18.104.22.168). Like the leaves, the fruit of the transformed tomato plants had elevated (2.4-fold) SPS activity. SPS over-expression in tomato fruit did not significantly change acid invertase, and only slightly reduced ADPglc ppase activity, but enhanced sucrose synthase activity by 27%. More importantly, the amount of sucrose unloaded into the fruit was considerably increased. Using [3H]- (fructosyl)-sucrose in in vitro unloading experiments with harvested 20-d-old fruit, 70% more sucrose was unloaded into the transformed fruits compared to the untransformed controls. Furthermore, the turnover of the sucrose unloaded into the fruit of transformed plants was 60% higher than that observed in the untransformed controls. Taken together, these results demonstrate that SPS overexpression increases the sink strength of transformed tomato fruit.
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