LPS activation is required for migratory activity and antigen presentation by tolerogenic dendritic cells

  1. Lookup NU author(s)
  2. Dr Amy Anderson
  3. David Swan
  4. Bethan Sayers
  5. Dr Rachel Harry
  6. Dr Angela Patterson
  7. Dr Alexei von Delwig
  8. Professor John Robinson
  9. Professor John Isaacs
  10. Dr Catharien Hilkens
Author(s)Anderson AE, Swan DJ, Sayers BL, Harry RA, Patterson AM, von Delwig A, Robinson JH, Isaacs JD, Hilkens CM
Publication type Article
JournalJournal of Leukocyte Biology
Year2009
Volume85
Issue2
Pages243-250
ISSN (print)0741-5400
Full text for this publication is not currently held within this repository. Alternative links are provided below where available.
Autoimmune pathologies are caused by a breakdown in self-tolerance. Tolerogenic dendritic cells (tolDC) are a promising immunotherapeutic tool for restoring self-tolerance in an antigen-specific manner. Studies about tolDC have focused largely on generating stable maturation-resistant DC, but few have fully addressed questions about the antigen-presenting and migratory capacities of these cells, prerequisites for successful immunotherapy. Here, we investigated whether human tolDC, generated with dexamethasone and the active form of vitamin D3, maintained their tolerogenic function upon activation with LPS (LPS-tolDC), while acquiring the ability to present exogenous autoantigen and to migrate in response to the CCR7 ligand CCL19. LPS activation led to important changes in the tolDC phenotype and function. LPS-tolDC, but not tolDC, expressed the chemokine receptor CCR7 and migrated in response to CCL19. Furthermore, LPS-tolDC were superior to tolDC in their ability to present type II collagen, a candidate autoantigen in rheumatoid arthritis. tolDC and LPS-tolDC had low stimulatory capacity for allogeneic, naïve T cells and skewed T cell polarization toward an anti-inflammatory phenotype, although LPS-tolDC induced significantly higher levels of IL-10 production by T cells. Our finding that LPS activation is essential for inducing migratory and antigen-presenting activity in tolDC is important for optimizing their therapeutic potential
PublisherSociety for Leukocyte Biology
URLhttp://dx.doi.org/10.1189/jlb.0608374
DOI10.1189/jlb.0608374
PubMed id18971286
Actions    Link to this publication
Share