Characterisation of Chlamydia psittaci isolated from a horse

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  2. Dr Meryl Lusher
  3. Dr Sam Richmond
Author(s)Wills JM, Watson G, Lusher ME, Mair TS, Wood D, Richmond SJ
Publication type Article
JournalVeterinary Microbiology
Year1990
Volume24
Issue1
Pages11-19
ISSN (print)0378-1135
ISSN (electronic)1873-2542
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This paper describes the isolation and characterisation of a strain of Chlamydia psittaci obtained from a nasal swab taken from a horse with serous nasal discharge. Initial isolation was achieved in cycloheximide-treated McCoy cell monolayers. Chlamydial inclusions stained by immunofluorescence either with a rabbit antiserum raised against C. psittaci or with a monoclonal antibody directed against the genus-specific lipopolysaccharide antigen were single and compact. They did not stain with iodine or with a monoclonal antibody reactive against Chlamydia trachomatis. The agent was re-isolated in the yolk sacs of embryonated hens eggs and designated N16. Identification of the agent was confirmed by electron microscopy. Unique plasmid DNA was prepared from a purified suspension of chlamydial elemenrary bodies (EBs), and analysed by electrophoresis through 1.0% agarose gels stained by ethidium bromide. This strain of C. psittaci grew relatively slowly in cycloheximide-treated McCoy cells, and the yield of elementary bodies during the course of one growth cycle was relatively low.
PublisherElsevier BV
URLhttp://dx.doi.org/10.1016/0378-1135(90)90046-X
DOI10.1016/0378-1135(90)90046-X
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