Characterisation of Chlamydia psittaci isolated from a horse
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- Dr Meryl Lusher
- Dr Sam Richmond
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| Author(s) | | Wills JM, Watson G, Lusher ME, Mair TS, Wood D, Richmond SJ |
| Publication type | | Article |
| Journal | | Veterinary Microbiology |
| Year | | 1990 |
| Volume | | 24 |
| Issue | | 1 |
| Pages | | 11-19 |
| ISSN (print) | | 0378-1135 |
| ISSN (electronic) | | 1873-2542 |
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| Full text for this publication is not currently held within this repository. Alternative links are provided below where available. |
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| This paper describes the isolation and characterisation of a strain of Chlamydia psittaci obtained from a nasal swab taken from a horse with serous nasal discharge. Initial isolation was achieved in cycloheximide-treated McCoy cell monolayers. Chlamydial inclusions stained by immunofluorescence either with a rabbit antiserum raised against C. psittaci or with a monoclonal antibody directed against the genus-specific lipopolysaccharide antigen were single and compact. They did not stain with iodine or with a monoclonal antibody reactive against Chlamydia trachomatis. The agent was re-isolated in the yolk sacs of embryonated hens eggs and designated N16. Identification of the agent was confirmed by electron microscopy. Unique plasmid DNA was prepared from a purified suspension of chlamydial elemenrary bodies (EBs), and analysed by electrophoresis through 1.0% agarose gels stained by ethidium bromide. This strain of C. psittaci grew relatively slowly in cycloheximide-treated McCoy cells, and the yield of elementary bodies during the course of one growth cycle was relatively low. |
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| Publisher | | Elsevier BV |
| URL | | http://dx.doi.org/10.1016/0378-1135(90)90046-X |
| DOI | | 10.1016/0378-1135(90)90046-X |
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