Toggle Main Menu Toggle Search

Open Access padlockePrints

Characterisation of Chlamydia psittaci isolated from a horse

Lookup NU author(s): Dr Meryl Lusher, Dr Sam Richmond

Downloads

Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


Abstract

This paper describes the isolation and characterisation of a strain of Chlamydia psittaci obtained from a nasal swab taken from a horse with serous nasal discharge. Initial isolation was achieved in cycloheximide-treated McCoy cell monolayers. Chlamydial inclusions stained by immunofluorescence either with a rabbit antiserum raised against C. psittaci or with a monoclonal antibody directed against the genus-specific lipopolysaccharide antigen were single and compact. They did not stain with iodine or with a monoclonal antibody reactive against Chlamydia trachomatis. The agent was re-isolated in the yolk sacs of embryonated hens eggs and designated N16. Identification of the agent was confirmed by electron microscopy. Unique plasmid DNA was prepared from a purified suspension of chlamydial elemenrary bodies (EBs), and analysed by electrophoresis through 1.0% agarose gels stained by ethidium bromide. This strain of C. psittaci grew relatively slowly in cycloheximide-treated McCoy cells, and the yield of elementary bodies during the course of one growth cycle was relatively low.


Publication metadata

Author(s): Wills JM, Watson G, Lusher ME, Mair TS, Wood D, Richmond SJ

Publication type: Article

Publication status: Published

Journal: Veterinary Microbiology

Year: 1990

Volume: 24

Issue: 1

Pages: 11-19

Print publication date: 01/07/1990

ISSN (print): 0378-1135

ISSN (electronic): 1873-2542

Publisher: Elsevier BV

URL: http://dx.doi.org/10.1016/0378-1135(90)90046-X

DOI: 10.1016/0378-1135(90)90046-X


Altmetrics

Altmetrics provided by Altmetric


Share