Heterologous production and secretion of Clostridium perfringens beta-toxoid in closely related Gram-positive hosts

  1. Lookup NU author(s)
  2. Dr Reindert Nijland
  3. Dr Leendert Hamoen
Author(s)Nijland R, Lindner C, van Hartskamp M, Hamoen LW, Kuipers OP
Publication type Article
JournalJournal of Biotechnology
ISSN (print)0168-1656
ISSN (electronic)1873-4863
Full text for this publication is not currently held within this repository. Alternative links are provided below where available.
The spore forming bacterium Clostridium perfringens is a widely occurring pathogen. Vaccines against C. perfringens type B and C are currently manufactured using beta-toxin secreted by virulent C. perfringens strains. Large-scale production of vaccines from virulent strains requires stringent safety conditions and costly detoxification and control steps. Therefore, it would be beneficial to produce this toxin in a safe production host and in an immunogenic, but non-toxic form (toxoid). For high-level expression of beta-toxoid, we cloned the highly active ribosomal rpsF promoter of Bacillus subtilis in a broad host range multicopy plasmid. In B. subtilis, we obtained high intracellular production, up to 200 microg ml(-1) culture. However, the beta-toxoid was poorly secreted. The employed rpsF expression system allowed using the same expression plasmids in other heterologous hosts such as Lactococcus lactis and Streptococcus pneumoniae. In these organisms secretion of beta-toxoid was ten times higher compared to the best producing B. subtilis strain. These results show the usefulness of the rpsF based broad host range expression system.
PublisherElsevier BV
NotesJournal Article Research Support, Non-U.S. Government Netherlands
Actions    Link to this publication

Altmetrics provided by Altmetric