The Bacillus secretion stress response is an indicator for alpha-amylase production levels
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- Dr Jan-Willem Veening
- Dr Sierd Bron
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| Author(s) | | Westers H, Darmon E, Zanen G, Veening JW, Kuipers OP, Bron S, Quax WJ, van Dijl JM |
| Publication type | | Article |
| Journal | | Letters in Applied Microbiology |
| Year | | 2004 |
| Volume | | 39 |
| Issue | | 1 |
| Pages | | 65-73 |
| ISSN (print) | | 0266-8254 |
| ISSN (electronic) | | 1472-765X |
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| Full text for this publication is not currently held within this repository. Alternative links are provided below where available. |
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| Aims: Overproduced α-amylases in Bacillus subtilis provoke a specific stress response involving the CssRS two-component system, which controls expression of the HtrA and HtrB proteases. Previously, the B. subtilis TepA protein was implicated in high-level α-amylase secretion. Our present studies were aimed at investigating a possible role of TepA in secretion stress management, and characterizing the intensity of the secretion stress response in relation to α-amylase production.Methods and Results: The expression of a transcriptional htrB–lacZ gene fusion, and the levels of α-amylase production were monitored simultaneously using tepA mutant B. subtilis strains. TepA was shown to be dispensable for secretion stress management. Importantly, however, the levels of htrB-lacZ expression can be correlated with the levels of α-amylase production.Conclusion: Our observations show that the secretion stress response can serve as an indicator for α-amylase production levels.Significance and Impact of Study: Conceivably, this stress response can be employed to monitor the biotechnological production of various secretory proteins by the Bacillus cell factory. |
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| Publisher | | Wiley-Blackwell Publishing Ltd. |
| URL | | http://dx.doi.org/10.1111/j.1472-765X.2004.01539.x |
| DOI | | 10.1111/j.1472-765X.2004.01539.x |
| PubMed id | | 15189290 |
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