The Bacillus secretion stress response is an indicator for alpha-amylase production levels

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  2. Dr Jan-Willem Veening
  3. Dr Sierd Bron
Author(s)Westers H, Darmon E, Zanen G, Veening JW, Kuipers OP, Bron S, Quax WJ, van Dijl JM
Publication type Article
JournalLetters in Applied Microbiology
Year2004
Volume39
Issue1
Pages65-73
ISSN (print)0266-8254
ISSN (electronic)1472-765X
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Aims: Overproduced α-amylases in Bacillus subtilis provoke a specific stress response involving the CssRS two-component system, which controls expression of the HtrA and HtrB proteases. Previously, the B. subtilis TepA protein was implicated in high-level α-amylase secretion. Our present studies were aimed at investigating a possible role of TepA in secretion stress management, and characterizing the intensity of the secretion stress response in relation to α-amylase production.Methods and Results: The expression of a transcriptional htrB–lacZ gene fusion, and the levels of α-amylase production were monitored simultaneously using tepA mutant B. subtilis strains. TepA was shown to be dispensable for secretion stress management. Importantly, however, the levels of htrB-lacZ expression can be correlated with the levels of α-amylase production.Conclusion: Our observations show that the secretion stress response can serve as an indicator for α-amylase production levels.Significance and Impact of Study: Conceivably, this stress response can be employed to monitor the biotechnological production of various secretory proteins by the Bacillus cell factory.
PublisherWiley-Blackwell Publishing Ltd.
URLhttp://dx.doi.org/10.1111/j.1472-765X.2004.01539.x
DOI10.1111/j.1472-765X.2004.01539.x
PubMed id15189290
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